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General Systems, see below for Inject and forget systems

Simplicity inside a bag.


Firstly let me say a big hello from the team here at MushBuddies.com.

My name is Mark and I will be guiding you from start to finish. You can email me for support anytime you get stuck. There are so many methods out there that it’s hard to know which one to choose. I am only covering four of these methods as not to confuse you more than needed.

For the beginner, using the same grow bags to incubate as well as fruit in removes most of the chances of contamination. This is because each bag will act as its own isolated grow chamber. By fruiting in the bag you will eliminate most chances of cake cross contamination also.

   These instructions are mindfully written to keep your costs and equipment needs to a minimum.

Our ready to use mushroom substrate bags come perfectly hydrated and conditioned so your mushrooms will grow big and strong.


Just by adding spores, warmth and a little patience you will have bags packed full of beautiful white mushroom mycelium ready to transfer to bulk substrate or fruit directly from the bag within a few weeks.

All items needed can be purchased in the MushBuddies.com Store

Four easy techniques


There are Four main methods that can be used with these bags, which are:


  • Incubate and fruit in the bag - Choose Rye Grain or Brf substrate. Ideal for beginners. Low cost and hassle free. No need for extra Tubs or Fruiting Chambers

  • PF Tek, Make a BRF cake - Ideal for the more experienced grower. A step on from the above allowing multiple cakes to be grown in the same Fruiting Chamber

  • Transfer Rye Grain or Crumbled Brf to a Bulk bag and fruit direct - Ideal for beginners. Very easy system allowing for massive harvests and no need to invest in Tubs or Fruiting Chambers

  • Mono Tub Bulk Grow- Layer Spawn with Bulk Substrate, Ideal for the more experienced grower. Big Yields grown in the Mono Tub of your Dreams

Do have a good read of the Guides and the Question and Answers section. If you are still stuck or need advice please get in touch.

What will i need?


The items that you will need from start to finish are as follows:

  • Isopropyl Alcohol spray
  • Kitchen towel
  • Gloves (for handling substrates and cakes)
  • Face Mask (when handling substrates and cakes)
  • One or more substrate bags
  • 1 Paper clip for each bag
  • Spore syringe (you can get these from www.SporeBuddies.com)
  • A heat mat with thermostat
  • A thermometer and or Hydrometer
  • Scissors
  • Sterilised vermiculite (for all methods apart from Growing direct in a bulk bag)
  • A spray bottle
  • A LED grow lamp (required if you are growing them in a concealed space)
  • Aluminium foil (required if using the Pf Tek cake method)
  • Perlite (required if using the  Pf Tek cake method)
  • Fruiting Chamber (required for Pf Tek method)

The following are only needed if you have chosen the Mono Tub bulk substrate method

  • Tub/Fruiting Chamber
  • Black bin liner
  • Bulk substrate
  • Polyester filter/cushion filter
  • Cling film

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Help and Assistance

World First: Inject and Forget Bags


Designed for Newcomers and Old school professionals. 

Fruiting Direct From the Bag


Would you like to keep you cost to a minimun?

PF Tek, Fruiting and Birthing a Cake


Feeling Confident and wanting to expand your Harvest with Multiple cakes?

Growing bulk from the Bag


Not tried but Growing before and want to do it the easy way.

Growing in a MonoTub


Would you like to see a Carpet of mushrooms



So the day is finally here but how do you harvest all these lovely fruits

Inject and Forget Bags A world First By www.MushBuddies.com


Hello my names Mark and I will be guiding you through this process step by step.

This Grow System works differently from any system produced for growing mushrooms so far. So please do not seek advice from anyone other than the team at MushBuddies.com. If you do need help along the way. Feel free to contact us here: MushBuddiesSales@Gmail.com and we will be more than happy to help. Please do not deviate from these instructions

Important Factors for this system to work well:

A. Blue spectrum led or florescent 6500k 

B. 26-27 DegC for germination of your Spores and Colonisation of the Substrate.

C: 25-26 DegC For Fruiting.


Temperatures lower than these will slow down your Grow. Temperatures hotter than 37 DegC may prevent spores from germinating and kill mycelium.

Opening the bag prematurely will allow contamination in.

Do buy a Thermostat Controlled Heat mat. Set it and then you can leave it to take care of your Grow.

Step 1 Inoculation of the Bag

This will be done with a Spore or liquid culture Syringe. Shake the Syringe well to mix up the solution inside

Take the alcohol swap and clean the black circular self healing injection port at the base of the bag.


You want to insert the syringe into the injection port and administer 5-10 ml of solution into the substrate block, saving the rest of the solution for later. Heat sterilise the needle replace the cap and keep the syringe in the fridge for next time 2-8 DegC.

Now go back to the bag and use your hands to massage the spores through out the substrate. The better you mix up the substrate the more even the growth.

Step 2 Reshape into a Fruiting Loaf

After mixing the spore solution and substrate well. Place the bag in front of you so the filter and injection port are facing you. Lay the bag over onto it left side. You will have one bottom corner and one top corner poking up in the air. 

Pick up the bag by these two raised corners and settle the substrate along the length of the bag by gently shaking side to side (left to right). 

Tape the probe in place (on one of the black section of the mat. Carefully place the bag on the heat mat. We have factory set the heat mat for you at 26-27 degC. Put the bag on top of the heat mat and the temperature probe in between the bag and the heat mat. check that the red light is on when temps are below 26 and the red light is off when temps are above 27c. call 07495515023 if you are unsure. 

 When you stand back and have a look, the bag will be on it's side. The substrate will be all broken up along the bottom.  The bag will look like a camping tent and the filter should be just above the substrate level. Nice job.

close the box loosly.

Step 3 Wait for life,

It will take the spores about 5-10 days to germinate and show the first signs of life. *Light must not shine on this bag until you are ready to fruit. You will see tiny white growth, this is the mycelium. 

Step 4 Allow the substrate to fully colonise so it all white

Do nothing to the bag. You are just waiting for the white mycelium to fully colonise the substrate. When the bag is fully white please wait 5 more days for the mycelium to grow strong. You may find a small patch of substrate under the filter patch that does not colonise. To remedy this  please pull the filter patch away from the substrate. But dont worry about this area too much. When the bag is fully white move to the next step.

Step 5 Turn On the lights its fruiting time.

Slide the light into position

Make sure the bag stays in the tent position. 

Turn on the lighting system. 24hrs a day is fine so no need for a timer. There is no need to drop temps either. so continue at 26-27degC


Step 6 Be patient and watch them grow.

Step 6 Be patient and watch them grow.

After about 5-14 days you will see tiny pins to forming. 

When the pins are getting to about 5-10mm

I want you cut 3 small finger nail sized holes in the  bag. One at either end and one in the top center of the tent. The end holes should be at or below the substrate surface level. These  small holes will allow Co2 to be expelled from the bag as your mushrooms grow.  Use scissors for this.  We have added Oxygen Release agents to help you out and the holes really help the process.


When 70% of the mushrooms have opened their caps you may now cut open one end of the bag and remove the Loaf to harvest your crop. Place the Loaf on top of the bag while you harvest. pull and twist the mushrooms free from the substrate. If there are smaller mushrooms. place the cake back into the bags and allow a few more days for the smaller mushrooms to mature. This has been your first flush. Congratulations. 

When all the mushrooms have been removed. The loaf may be soaked for 12HRS in sterile water(TAP WATER) To do this. Place the loaf back into the bag and fill the bag up with water. you may have to close some holes in the bag. After 12 hrs. Drain away the water and place on the heat pad and under the light for 2nd, 3rd and fourth flushes etc. fruiting at 26-27 DegC. Please do not add vermiculite to the loaf. There is no need. 

Fruiting Brf AND GRAIN Cakes Directly From the Bag

Prepare your Surface


Ok so your products have arrived and you’re really excited. Take a moment to calm down and think about the steps ahead. You’ll need to give yourself time, and a clean area to work. This step is required for all of the Growing Methods and should become part of your standard procedure. Choose a room where there are no Drafts or Bad Smells. Close all windows and doors and make sure you have all items you need inside the room that your are working in.

  • Alcohol spray
  • Paper Towel for wiping down
  • Substrate Bags
  • Spore Syringe
  • Wind Proof Lighter for needle sterilisation
  • Marker pen for species and date info on the bag
  • Etc

Ladies tie your hair out of the way. Everybody roll your sleeves up and wash your hands well. Use Isopropyl Alcohol to clean the surface you are to work on. Open the Box containing the bags of sterilised mushroom substrate and remove the white outer protective bags. Check for contaminated or punctured bags. If found please contact us immediately and we will provide a replacement or refund. Place the mushroom substrate bags on the surface.

Prepare your Bags


Both the Rye Grain and the BRF bags will work best if a little air is drawn into the bags through the filter so the contents can be gently mixed up. BRF bags are best when the contents have been crumbled up into a soft cookie crumble. During the sterilisation process tiny air pockets inside the BRF/VERMICULITE mix are crushed and squashed by the high pressures inside the steriliser. By crumbling up the mix you will add the air back and this will really speed up the colonisation of the substrate by the mycelium.

The best way i have found to do this is to hold the bag up as if it were a bag of crisps that you were about to open.

*It is best to use one hand to pinch the bag just to one side of the filter (white square) and with the other hand pinch the back of the bag just behind the filter. Now slowly and carefully pull the filter side away from the back of the bag. By pulling the filter away from the back of the bag you will draw filtered air into the bag*

This will give you room to break up the BRF block into the crumble the mycelium loves. Do the same for the Rye Grain bags. The grains will easily separate and will be free in the bag.



Now open the spore syringe packet. Remove the blue syringe protector and screw in the needle but leave the needle cap on the needle. Use the alcohol swab that comes with each bag to wipe the round black self healing injection ports. Pick up the syringe and remove the needle cap. *DO NOT TOUCH THE NEEDLE WITH YOUR FINGERS, ALCOHOL WIPES OR ANYTHING ELSE. IF YOU DO, HEAT THE NEEDLE TIP TO RED HOT WITH THE WINDPROOF LIGHTER AND ALLOW TO COOL. IT WOULD ALSO BE A GOOD IDEA TO HEAT STERILISE THE NEEDLE BEFORE INJECTING EACH BAG AND BEFORE PUTTING AWAY.  You want to pierce the injection port fully with the needle and inject about 2ml of solution as you pull the needle out, this should leave a line of spore solution right through the centre of each bag. You will see the first signs of growth from this injection site. If you have any solution left you can Heat sterilise the needle and put the cap back on and store in the fridge at 2-8degC for future use. When you have completed this step you can put the mushroom substrate bags back into the packing box and use the box to incubate them.




Place your inoculated Bags of substrate back into the bubble lined packing box and position the box on the heat mat. Somewhere clean and out of the way. Place your thermometer inside the box. Put the Temperature probe of the thermostat directly under a bag. This positioning of the thermostat prob is important.

  White, fluffy-looking mycelium should start to appear between 4-14 days after inoculation with spores, spreading outward from the inoculation site like fine silk. Note: Mushbuddies.com products are double sterilised to prevent contamination. However your spore syringes can not be sterilised. Watch out for any signs of contamination during incubation, including strange colours and smells. Dispose of any suspect Bags immediately. Do not open the mushroom substrate bag just put it straight in the bin. If you’re unsure about whether a bag is contaminated, always err on the side of caution even if the substrate is otherwise healthily colonised.

7 Days after you see mycelium starting to grow, we recommend that you break up the Brf substrate bag contents into tiny bits leaving no clumps or lumps and gently break up the rye grain so its loose.

Do not worry this will not damage the mycelium. Instead this allows the mycelium to colonise much faster. Break up the bags contents by using your hand on the outside of the bag. To finish, gently flatten the surface of the substrate ready for the vermiculite casing layer. Do not open the bag at this stage. Wait another 7 days, if all goes well, you should have Bags full of white mycelium and you are ready for the next step. For all other methods Click here

Casing Layer and Soak


In the previous step you gently flattened the surface of the mycelium. This was done so when you add the hydrated vermiculite casing layer (contamination protection and micro climate generator)) you will get a full and even pin set leading to maximum harvest weight.

You should add your sterilised vermiculite now.

To add the vermiculite simply cut the top off the colonised substrate bags and sprinkle around 5mm of vermiculite to the top of the fully colonised mushroom substrate. Now fold down the top 2 inches of the bag and seal with paperclips, pegs or something similar.

Put the bag back in a dark warm place to consolidate for 7 more days. After 7 days the mycelium will have grown up through the vermiculite and you are ready to Fruit. One last step for this system, you need to Soak the cakes by boiling up a cup of water for each bag you want to fruit, allow the water to cool before use by covering each cup and leaving to one side.

Open one bag at a time and gently pour the cup of cooled water into each bag then seal with a paperclip or similar as you go. Once the sterilised water has been added to all the bags, leave in a cool place. After 12 hours you need to drain the bags, this can be done by laying the tops of the bags into the sink so as all the water drains away from the cake. Leave for 15 minutes then carefully drain the water from the bag. The Vermiculite will stay attached to the cake where it is needed and dont worry if some falls down the sides. 

So well done.

You now have bags of silky white mycelium with a 5mm layer of vermiculite on top. The bags have been drained fully and you are ready to fruit.

Fan and Air


The bags will now need a source of light. Light causes the mycelium to start to fruit. Led lights are compact ,low cost and best of all generate almost no heat. Normal light bulbs will need to be placed a distance from your grow bags or you will cook the bags. Led lights can be in direct contact with the bags meaning a small space can be used to house your grow area.

Light is a Pinning trigger as is fresh air and evaporation. Light also shows the mushrooms which way is up so i recommend the light source be directly above your bags. 12 Hours on 12 Hours off. If the light source is off to one side, your mushrooms will lean in that direction.

To add fresh air and moisture for evaporation you should open all the bags at least once a day (three times a day is best) and spray a small squirt of fine water mist against the inside walls of the bag and fan the top of the bag for 40 seconds with a piece of cardboard. Doing so will allow fresh air in and carbon dioxide out. Adding fine mist and fanning completes the evaporation process.

Now again, close the bag by rolling over the top 2 inches of the bag and adding your paper clip.

The temperature of the mat needs to be lowered to 21-23degC.

From this point on, open the top of the bag one to three times a day. Mist and fan fresh air into the bag for about 40 seconds.

DO NOT SPRAY THE WATER DIRECTLY ONTO THE SUBSTRATE SURFACE. ONLY ON THE INSIDES OF THE BAGS. You want to see a very fine dew on the vermiculite's surface with out any pools of water, ease off on the spray if water starts to pool.

Continue this until tiny pins appear or two weeks has passed (Repeat the Soak if pins fail to show). A top tip is to keep the bags slightly damp for the first 7 days or until your pins are 10mm tall. You want to aim for a fine Dew over the surface of the cake. Each day after this you will need to open the bags and spray a little less fine mist on the inside of the bags while continuing to Fan the top for 40 seconds. This has to be repeated once to three times a day until the mushrooms are mature and ready for harvesting. For most of you this will be just as the veil breaks away from the stem. If you wait to long the mushroom will drop its spores and these spores will clog the surface and lead to contamination. See Harvesting

Pf Tek, Fruiting a birthed cake in a fruiting chamber

Prepare your fruiting chamber


Wooow You have four fully colonised Brf cakes and you would like to fruit all these cakes in the one fruiting chamber.

Until we Launch the "TubbBuddies"  range of fruiting chambers, you will have to make your own. Do not worry tho its not too hard.

Step 1 Find a clear tub with a clear lid. A 20Litre storage tub will work great.

Step 2 Draw a line all round the base of the box at 2" high. Get your self a drill and a 5mm bit and drill holes all along this line. At least every 2cm. Do the same along the top of the tub. These are for air flow.

Step 3 Pour your 3Litres of Sterilised Perlite into your tub. The perlite should come higher than your line of drilled holes and will filter the air as its exchanged.

Step 4 Pour Sterilised water into the tub to wet the perlite. Bring the water level just shy of you line of holes.

Step 6 Add Parcel tape to the line of holes at the top of the tub.  We keep these closed off until the pins are 10mm tall.

Step 7 Evenly place 4x 5inch square pieces of aluminium foil on the Perlite.

Step 8 Set your light on a 12hr timer. This can sit on the closed lid if its LED.

Prepare you Brf cakes

Both the Rye Grain and the BRF bags will work but Brf is preferred for this method as the fully colonised cakes are stronger and wont crumble during handling. 

You will need:

  • Scissors
  • Sterilised water
  • 1000ml of Sterilised DRY Vermiculite

You need to Soak the cakes by boiling up a cup of water for each bag you want to fruit, allow to cool before use. Cover each cup and leave to one side until cool.  Open one bag at a time and gently pour the cup of cooled water into each bag then seal with a paperclip or similar as you go. Once the sterilised water has been added to all the bags, leave in a cool place. After 12 hours you need to drain the bags, this can be done by laying the tops of the bags into the sink so as all the water drains away from the cake. Leave for 15 minutes then carefully drain the water from the bag.

Once fully Drained the cakes can be removed from the bag. 

Take a pair of scissors and carefully cut the bag down its length. With a gloved hand removed the cake and place it into the bowl of DRY Vermiculite. Roll the cake around in the Vermiculite good and proper, aim for complete covering of vermiculite for a great pinset.